亚洲日本精品国产第一区二区_香港经典a毛片免费观看hd_欧美放荡的少妇_亚洲va久久久噜噜噜久久_少妇装睡让我滑了进去

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>HTB-119NCI-H69 H69細(xì)胞,人肺小細(xì)胞肺癌細(xì)胞

NCI-H69 H69細(xì)胞,人肺小細(xì)胞肺癌細(xì)胞

簡(jiǎn)要描述:NCI-H69 H69細(xì)胞,人肺小細(xì)胞肺癌細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):HTB-119
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:1812

產(chǎn)品分類

Product Category

詳細(xì)介紹

NCI-H69 [H69]細(xì)胞,人肺小細(xì)胞肺癌細(xì)胞

ATCC® Number:HTB-119™ Price:$279.00
Designations:NCI-H69 [H69]Depositors:AF GazdarBiosafety Level:1Shipped:frozenMedium & Serum:Seaggregatese PropagationGrowth Properties:suspension, multicellOrganism:Homo sapiensMorphology:floating aggregates Source:Organ: lung Disease: carcinoma; small cell lung cancerPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.NCI-H69 [H69]細(xì)胞,人肺小細(xì)胞肺癌細(xì)胞Receptors:insulin-like growth factor II (IGF II)Tumorigenic:YesOncogene:myc +; myb +; fes +; fms +; raf +; ras +DNA Profile (STR):CSF1PO: 10, 12 D13S317: 12 D16S539: 11 D5S818: 11, 13 D7S820: 9 THO1: 8, 9 TPOX: 10 vWA: 16, 17 Amelogenin: XYCytogenetic Analysis:modal number = 76 to 78; range = 40 to 87This is an aneuploid human male cell line. Monosomy of many of the normal chromosomes is noted as well as bisomy in this subtetraploid cell line; however, translocations and deletions involving many of the missing chromosomes are noted, and these chromosomal rearrangements appear to be stable and generally paired. Twelve marker chromosomes were identified including: der(16)t(1;16)(q21;q23), der(22)t(4;22)(q12;q13), der(12)t(11;12)(q23;p12), del(17)(p11), der(19)t(5;19)(?q21;q13) and others.Isoenzymes:AK-1, 1 ES-D, 2 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 2 PGM3, 1Age:55 years adultGender:maleEthnicity:CaucasianComments:This cell line is aneuploid, will form colonies in soft agar and retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics. The cells grow in aggregates, thus cell counts are not accurate. The cells stain positively for cytokeratins. The line can be adapted to grow in shaker flask or spinner flask systems. The N-myc gene is amplified, and there is expression of the mRNA and protein. C-myc mRNA, but not protein, is expressed at a low level. There is expression of c-myb, v-fes, v-fms, c-raf 1, Ha-ras, Ki-ras and N-ras mRNA.Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°CSubculturing:Protocol: Attached cells can be detached by shaking flask Allow aggregates to settle to the bottom of the flask, remove and discard the supernatant medium. Add fresh medium, disperse cells by gentle pipetting and dispense into new flasks. Do not break down aggregates. Subculture every 6 to 8 days.                         NCI-H69 [H69]細(xì)胞,人肺小細(xì)胞肺癌細(xì)胞Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 times weeklyPreservation:Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperatureRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020derivative:ATCC CRL-11351purified DNA:ATCC HTB-119DReferences:1805: Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed:664620122250: Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 253691722446: Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 838014122465: Broers JL, et al. Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines. J. Cell Sci. 91: 91-108, 1988. PubMed: 247308622869: Rygaard K, et al. Expression of myc family oncoproteins in small-cell lung-cancer cell lines and xenografts. Int. J. Cancer 54: 144-152, 1993. PubMed: 838670723036: Gazdar AF, et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 610815623037: . . Cancer Res. 40: 4556-4563, 1980.23056: Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 298525723057: Gazdar AF, et al. Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties. Cancer Res. 45: 2924-2930, 1985. PubMed: 298525823065: Kiefer PE, et al. Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res. 47: 6236-6242, 1987. PubMed: 282402823080: Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 215937024389: . . Lung Cancer 4: 155-161, 1988.32276: Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 939376032488: Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
男女啪啪高潮激烈免费版| 色偷偷av男人的天堂京东热| 人人妻人人澡人人爽人人dvd| 男人进入女人下部视频| 精品人妻无码一区二区三区性| 中文字幕国产精品综合| 香蕉久久人人爽人人爽人人片av| 18禁免费无码无遮挡不卡网站| 午夜嘿嘿嘿在线观看| 欧美老熟妇欲乱高清视频| 99精品视频在线观看免费| 国产精品99久久99久久久动漫| 成人网站免费看黄a站视频| 无码国产福利av私拍| 国产三级韩国三级日产三级剧情| 人妻暴雨中被强制侵犯在线| 精品国产一区二区三区av 性色| 美女视频黄又黄又免费| 成人无码区免费a片在线软件| 日本一区二区三区精品| 欧美午夜刺激影院| 国产精品二区一区二区aⅴ污介绍| 久久午夜神器| 全免费a级毛片| 熟女俱乐部五十路六十路av| 欧美精品在线观看| 亚洲国产精品无码久久一区二区| 色九月亚洲综合网| 久久国产精品99国产精| 国产精品精品一区二区| 久久人人爽人人爽人人片av麻烦| 国产成人久久av免费看| 精品少妇人妻av免费久久久| 中文字幕av无码不卡| 极品熟妇大蝴蝶20p| 国产亚洲精品资源在线26u| 男人添女人下部高潮全视频 | 精品人妻一区二区三区中文久久 | 国精产品一区二区三区有限公司| 欧美最猛黑人xxxx黑人猛交98| 亚洲国产av无码精品|