亚洲日本精品国产第一区二区_香港经典a毛片免费观看hd_欧美放荡的少妇_亚洲va久久久噜噜噜久久_少妇装睡让我滑了进去

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2557Panc 05.04 人胰腺癌細(xì)胞

Panc 05.04 人胰腺癌細(xì)胞

簡要描述:CRL-2557 Panc 05.04 人胰腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2557
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-12
  • 訪  問  量:1812

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2557 Panc 05.04 人胰腺癌細(xì)胞

ATCC® Number: CRL-2557™ Price: $417.00

Designations: Panc 05.04

Depositors: EM Jaffee

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreas

Disease: adenocarcinoma

Cellular Products: cytokeratins 7 and 18 [50655]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2557 Panc 05.04 人胰腺癌細(xì)胞

Isolation: Isolation date: May 5, 1995

Tumorigenic: Yes

Oncogene: K-ras +

Antigen Expression: MHC class I +; MHC class II - [50655]

Blood type B; Rh+

Age: 77 years adult

Gender: female

Ethnicity: White

Comments: Panc 05.04 is a pancreatic adenocarcinoma epithelial cell line derived, in 1995, from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.

The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655]

The cells have a reported plating efficiency of 100%. [50655]

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 20 Units/ml human recombinant insulin, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:5 is recommended

Medium Renewal: Add media once per week. Fluid change one to two times per week.

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 46 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

CRL-2557


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
免费在线观看av播放| 青草影院内射中出高潮| 精品亚洲女同一区二区| 美女内射视频www网站午夜| 久久精品国产亚洲av无码娇色| 蜜桃传媒一区二区亚洲av| 新婚人妻不戴套国产精品 | 亚洲精品无码永久在线观看性色| 专干老肥熟女视频网站| 精品欧美一区二区三区久久久| 中日韩精品视频在线观看| 久青草无码视频在线播放| 久久久亚洲精品无码| 久久久噜噜噜久久中文字幕色伊伊| 国内精品伊人久久久久av影院| 亚洲精品无码久久久久牙蜜区| 久久99精品国产精品| 337p人体粉嫩胞高清视频| 免费做a爰片久久毛片a片| 国产精品久久婷婷六月丁香| 少妇邻居内射在线| 国产成人乱码一二三区18| 亚洲av永久无码精品网站在线观看| 女同互玩中文字幕久久| 欧美成人精品一区二区综合| 国产操逼视频| 亚洲成av人在线观看天堂无码 | 亚洲熟妇无码av另类vr影视| 久久久日韩精品一区二区| 亚洲欧美国产精品久久久久久久 | 一区二区三区人妻无码| 欧美激情精品久久| 国产精品日本一区二区不卡视频| 亚洲av永久无码精品网站在线观看| 国内揄拍国内精品| 在线国产69自拍视频| 欧美内射深喉中文字幕| 日本一区二区三区黄色| 亚洲av日韩av永久无码绿巨人 | 国产国产精品人在线视| 免费看国产v不卡网站|