亚洲日本精品国产第一区二区_香港经典a毛片免费观看hd_欧美放荡的少妇_亚洲va久久久噜噜噜久久_少妇装睡让我滑了进去

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>動物卵巢細(xì)胞>CRL-9618CHO-K1 倉鼠卵巢細(xì)胞亞株

CHO-K1 倉鼠卵巢細(xì)胞亞株

簡要描述:CRL-9618 CHO-K1 倉鼠卵巢細(xì)胞亞株,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號:CRL-9618
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2404

產(chǎn)品分類

Product Category

詳細(xì)介紹

CHO-K1 倉鼠卵巢細(xì)胞亞株

1957年,Puck TT從成年中國倉鼠卵巢的活檢組織建立了CHO細(xì)胞,CHO-K1是CHO的一個亞克隆。CHO-K1的生長需要

培養(yǎng)條件:F12K培養(yǎng)基(SIGMA,貨號N3520,添加NaHCO3 2.5g/L),90%;優(yōu)質(zhì)胎牛血清,10%


動物種別:中國倉鼠

性別:雌

組織來源:卵巢

形態(tài):上皮細(xì)胞

傳代方法的介紹   1:4-1:8

支原體檢測   陰性

以下是此細(xì)胞ATCC

CHO-K1 (ATCC® CCL-61™)
CHO-K1 倉鼠卵巢細(xì)胞亞株

Organism Cricetulus griseus, hamster, Chinese

Tissue  ovary

Product Format  frozen

Morphology  epithelial-like

Culture Properties  adherent

Biosafety Level  1

Gender  female

Applications

This cell line is suitable as a transfection host.

Storage Conditions  liquid nitrogen vapor phase

Karyotype  Chromosome Frequency Distribution 50 Cells: 2n = 22. Stemline number is hypodiploid.

Derivation

The CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957.

Clinical Data

female

Virus Susceptibility  Vesicular stomatitis, Orsay (Indiana)

Vesicular stomatitis, Glasgow (Indiana)

Getah virus

Virus Resistance

poliovirus 2; modoc virus; Button Willow virus

Comments

The cells require proline in the medium for growth.

Complete Growth Medium  The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w

) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended

Medium Renewal: Once or twice between subculture

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
午夜性色一区二区三区不卡视频| 亚洲午夜成人片| 中文字幕有码视频日韩| 精品av熟女一区二区偷窥海滩| 亚洲不卡av不卡一区二区| 亚洲欧美中文字幕在线一区| 国产精品亚洲一区二区三区| 最新黄色影院不卡视频| 国产又黄又硬又粗| 青娱国产区在线| 日本一道高清一区二区三区| 久久精品国产亚洲avapp下载| 久久精品久久久久观看99水蜜桃| 99久久婷婷国产综合精品电影| 综合久久青青草免费观看视频| 久久久综合九色综合| 精品久久久中文字幕人妻| 亚洲精品乱码久久久久久不卡| 亚洲av中文无码字幕色三| 男女无遮挡啪啪啪国产| 欧美xxxxx高潮喷水麻豆 | 啪啪视频免费看一区二区| 久久国产精品无码网站| 欧美激情一区二区三区在线| 老师粉嫩小泬喷水视频90| 被三个男人绑着躁我好爽| 久久精品午夜福利| 同桌上课脱裙子让我帮他自慰| 日本一道一区二区视频| 人妻熟女一区| 狠狠色婷婷久久一区二区| 久久精品国产99久久丝袜| 欧美三级午夜理伦三级| 欧美熟妇另类久久久久久多毛| 欧洲lv尺码大精品久久久| 日产亚洲一卡2卡3卡4卡网站| 日本在线观看| 曰韩无码av一区二区免费| 久久精品道一区二区三区| 久久亚洲精品无码av红樱桃| 亚洲精品成人a在线观看|